Ariane Falone GOUMTSAUniversity of Dschang, Cameroon
Title: Cardioprotective effect of the seed ethanolic extract of Aframomun pruinosum Gagnep. (Zingiberaceae) against isoproterenol-induced cardiac hypertrophy in rat
Background: Pathological Cardiac?Hypertrophy?is?the?enlargement?of?the?myocardium?in response?to chronic mechanical and neurohormonal stress that can finally lead to heart failure, a condition associated with high morbidity and mortality.
Aims: The objective of this study was to evaluate the ability of the ethanol extract of Aframomum pruinosum (EEAP), a plant used in Cameroonian folk medicine to manage cardiovascular ailments, to prevent the development of cardiac hypertrophy in rats.
Methods: Isoproterenol (0.3mg/kg/day) was subcutaneously injected to adult male Wistar rats alone or concomitantly with EEAP (37.5, 75,or 150 mg/kg, per os) or propranolol (dose, per os) for 7 consecutive days. Systolic blood pressure (SBP), diastolic blood pressure (DBP), and heart rate (HR) were recorded at baseline and at the end of the treatment. The heart was harvested at the end of the experiment and weighed. Sections of the cardiac tissue were stained with hematoxylin and eosin or with Masson trichrome. Myeloperoxidase (MPO), superoxide dismutase (SOD), catalase, and reduced glutathione (GSH) were assessed in cardiac homogenates. Chemical profiling of EEAP was done by gas chromatography-mass spectrometry (GC-MS).
Results: The administration of isoproterenol induced decreased in SBP and DBP while a significant (p <0,001) increase in cardiac mass, cardiomyocyte surface, and MPO was observed. All these alterations were significantly prevented (p < 0,001) by EEAP. In addition, EEAP inhibited immune cell infiltration and cardiac fibrosis elicited by isoproterenol. Although not significant (p > 0,05), isoproterenol reduced SOD and catalase activity associated with a reduced GSH levels which were also prevented by EEAP. GC-MS of EEAP showed that their possesses 75,5% monoterpenes, Reducing Sugar 2,28% Biguanides 0,95%,Tanins 0,87%, Flavonoides 0,48%, and 18,5% of sequiterpenes mainly Nerolidol, which have been reported as anti-oxidant, anti-inflammatory and anti-cancer.
Conclusion: EEAP possesses cardioprotective effect against isoproterenol-induced cardiac hypertrophy that may result from its antifibrotic, anti-inflammatory, and antioxidant properties which may be accounted to Nerolidol
GOUMTSA Ariane is a PHD student in cardiovascularphysiology and phytopharmacology at the Dschang University, Cameroon. She is 31 years old, married and has one child. She is Cameroonian. She is Member of the Cameroon Forum for Biological Sciences (CAFOBIOS). She has participated to many national and international conferences (CAMES, in DAKAR). She possessed 02 publications and many Scientifics communications. She has also obtained a price on poster presentation at 24th Annual conference of Cameroon Biosciences Societies (CBS). She has been selected by the CASEG Programm (DAAD) from the University of Cologne to improved knowledge in histology and immunohistochemistry in DAKAR.