Mohammad Esmaeil AkbariShahid Beheshti University of Medical Sciences, Iran
Title: Radiobiological effects of wound fluid on breast cancer cell lines and human derived tumor spheroids in 2D and microfluidic culture
Intraoperative radiotherapy (IORT) could abrogate cancer recurrences, but the underlying mechanisms are unclear. To clarify the effects of IORT-induced wound fluid on tumor progression, we treated breast cancer cell lines and human-derived tumor spheroids in 2D and microfluidic cell culture systems, respectively. The viability, migration, and invasion of the cells under treatment of IORT induced wound fluid (WF-RT) and the cells under surgery-induced wound fluid (WF) were compared. Our findings showed that cell viability was increased in spheroids under both WF treatments, whereas viability of the cell lines depended on the type of cells and incubation times. Both WFs significantly increased sub-G1 and arrested the cells in G0/G1 phases associated with increased P16 and P21 expression levels. The expression level of Caspase 3 in both cell culture systems and for both WF-treated groups was significantly increased. Furthermore, our results revealed that although the migration was increased in both systems of WF-treated cells compared to cell culture media-treated cells, E-cadherin expression was significantly increased only in the WF-RT group. In conclusion, WF-RT could not effectively inhibit tumor progression in an ex vivo tumor-on-chip model. Moreover, our data suggest that a microfluidic system could be a suitable 3D system to mimic in vivo tumor conditions than 2D cell culture.
Prof Mohammad Esmaeil Akbari is the head of Cancer Research Center and Comprehensive Cancer Control Center at SBMU, a board member of EURAMA (Europa-Asian Breast Cancer Association), member of Iranian Association of Surgeons, member of American Association of Surgeons, President of the Association of World with No Tobacco. He was the chancellor of Isfahan University of Medical Sciences and deputy for health at Ministry of Health and Medical Education. He has published a considerable number of articles and books in the field of cancer management.