Xuan Hai DOVietnam Military Medical University, Vietnam
Title: Evaluation of fresh umbilical cord arteries as a novel substitute for vascular bypass grafting
Small vascular diameter grafts (d<6 mm) have been developed based on synthetic vascular prostheses, autologous bypass surgery, and decellularized umbilical vessels are currently the best solutions for these groups. In this present study, we evaluated the possibility of pre-treated fresh umbilical cord arteries (FUCA) serving as a vascular graft without using any immunosuppression process. FUCA (n=8) were collected and pre-treated with a special cocktail of the sterilizing solution, then implanted in the abdominal aorta below the kidney of Wister rats (n=8). The tests were performed at the end of the implantation process (n=8), on day 3 (n=5), on day 30 (n=3), and on day 90 (n=1) after transplantation. 100% of rats (8/8) were positive with the revascularization and perfusion assessments, with blood pressure values of about 110/70 mmHg. Doppler ultrasound indicated good circulation with the separately three echogenic layers corresponding to three layers of the arterial wall at three time-points assessment. BBB hill climb test during the first week resulted in the highest point of 21, with a positive response to the thermal and pain stimulation. The blood biochemical values and whole blood cell count were not significantly different before and after the transplantation (p>0.05). Histological analysis of the grafts showed that there was no calcification presenting, and the rate of SMA (%), B, T lymphocytes, and macrophages were not increased compared to the control. To evaluate the immunogenicity of the grafts, subcutaneous implantation (SI) was performed, and the results showed that the size of FUCA after 3, 7, and 30 days were similar to the one before implantation. In conclusion, FUCAs retained their structure and function properties after transplantation on rats without using any immunosuppression conditions and could be a potential source for readily accessible small diameter vascular grafts.
Dr. Xuan Hai DO has completed his PhD. in thoracic surgery-cardiovascular in the year 2019 from Vietnam Military of Medicine University. He is now the Deputy Head of the Practical and Experimental Surgery Department and works as a Doctor at Cardiothoracic Surgery Department - 103 Hospital. He has published more than 50 papers and technical innovations in specialized journals and has been serving as an invited speaker at reputed international conferences. His research areas include heart valve surgery, organ transplantation, and experimental surgery. He has been participating in many types of research on organ transplantations such as heart transplants, pancreas transplants, lung transplants, small intestine transplants... Research on new models of liver transplantation and experimental uterine transplantation is underway. He is also the main member engaged in research on medical education, especially surgical medicine. At present, he is managing and participating in several projects involving umbilical cord artery in vascular graft, 3D bio-printing of the blood vessel, and applying stem cell products to the treatment of stroke.