Rishu

India, India

Title: Evaluation of direct antimicrobial susceptibility testing from positive bactec blood culture bottles

Abstract

Background: Conventional methods for isolation and antibiotic susceptibility reports of bacterial isolates from positive blood culture bottles takes at least 48 hours. Direct

antibiotic susceptibility testing (AST) helps to reduce the turnaround time (TAT) by 24 hours which will be useful in early initiation of appropriate antimicrobial to reduce

morbidity and mortality.

Aim & Objectives: To evaluate the performance of direct AST from positively flagged BACTEC blood culture bottles and its comparison to conventional AST.

Methods: A total of 356 blood culture bottles that were positively flagged on BACTEC 9120 were processed. Direct AST was performed from positive blood culture bottles.

Bacterial isolates were identified using standard microbiological methods and tested against a wide spectrum of antimicrobial agents using the Kirby Bauer’s disc diffusion

method following the Clinical & Laboratory Standards Institute (CLSI) guidelines. Direct and conventional AST results were compared and Categorical agreement (CA)

with various errors were evaluated.

Results: On direct Gram staining, 107 samples showed Gram negative bacilli and 105 showed Gram positive cocci and 144 samples showed contaminants. A total of 1486

organism-antibiotic combinations were evaluated, out of which 1438 (96.76%) combinations showed CA which was extremely satisfactory. The categorical disagreement was found only in 48 (3.23%) of organism-antibiotic combinations; out of which majority were major error 23 (1.54%) followed by minor error 17 (1.14%) and very major error 8 (0.53%).

Conclusions: The present study showed good concordance between the two methods and suggests the use of direct AST to reduce TAT by 24 hours for early initiation of therapy in patients with blood stream infections.

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4th International Conference on Cardiology

Rishu

Title: Evaluation of direct antimicrobial susceptibility testing from positive bactec blood culture bottles

Abstract

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